I₂
Starch Test
Reagent: Iodine solution.
Method: Add a few drops of iodine to the food sample.
Positive result: Yellow-brown iodine turns blue-black.
Conclusion: Starch is present.
B
Glucose Test
Reagent: Benedict’s solution.
Method: Add Benedict’s solution and heat in a warm water bath.
Positive result: Blue changes to green, yellow, orange or brick-red depending on glucose amount.
Conclusion: Reducing sugar such as glucose is present.
Cu
Protein Test
Reagent: Biuret test: sodium hydroxide and copper sulphate solution.
Method: Add sodium hydroxide, then a few drops of copper sulphate and mix gently.
Positive result: Blue changes to purple or violet.
Conclusion: Protein is present.
EtOH
Lipid Test
Reagent: Ethanol and water, or brown paper for a grease-spot test.
Method: Shake food with ethanol, then add water.
Positive result: Milky white emulsion forms.
Conclusion: Lipid is present.